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sRNA Annotations

2nd structure
(RNAfold)
sRNAG0013Get Sequence
Gene Name dsrA
Synonyms ECK1952;b1954;
Position 2023251.....2023337
Length 87 bp
Strand reverse
Gene Class DsrA RNA; riboregulator of RpoS and H-NS production
Species Escherichia coli K12 MG1655
Evidence Inferred from experiment;Inferred from direct assay;
Source regulonDB

Genome position

Structural RNA alignment
(RNALogo)
Get alignment sequences

Descriptions


    "DsrA RNA activates production of RpoS (the stationary phase sigma factor), stimulating this process during log phase growth at low temperature (Sledjeski DD,1996). DsrA acts to inhibit H-NS translation (Lease RA,1998). DsrA RNA affects biosynthesis of capsular polysaccharide via increased production of the activator RcsA, due to inhibition of H-NS mediated transcriptional silencing (Sledjeski D,1995). Additional genes induced by DsrA have been identified by microarray analysis and include acid resistance genes (Lease RA,2004). DsrA regul.....

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References

Sledjeski DD, Gupta A, and Gottesman S (1996) "The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli." EMBO J 15 (15)  PubMed
Loewen PC, Hu B, Strutinsky J, and Sparling R (1998) "Regulation in the rpoS regulon of Escherichia coli." Can J Microbiol 44 (8)  PubMed
Repoila F, and Gottesman S (2003) "Temperature sensing by the dsrA promoter." J Bacteriol 185 (22)  PubMed
MassĂ© E, Escorcia FE, and Gottesman S (2003) "Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli." Genes Dev 17 (19)  PubMed
Klauck E, Böhringer J, and Hengge-Aronis R (1997) "The LysR-like regulator LeuO in Escherichia coli is involved in the translational regulation of rpoS by affecting the expression of the small regulatory DsrA-RNA." Mol Microbiol 25 (3)  PubMed
more..

Literature Records


Species Conditions Experiment Type Regulation PMID
Escherichia coli at low temperatures   induce PubMed
Escherichia coli at low temperatures   induce PubMed

Computational Records


Gene NameStartEndStrand
IS095 2023239 2023336 reverse

score promoter_score=62.233;terminator_score=-15.71
Note GenBank_annotation:dsrA sRNAgene
Methods "profile-based search method(profile search program, pftools2.2) to identify putative promoters in the E. coli genome."
Species Escherichia coli K12 MG1655
Sequence Get Sequence
PMID PubMed

Comparative Genomics by UCSC Genome Browser

Network Visualization

Regulators/sRNAs


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sRNAs/Targets


sRNA Expression Profiles

TitleF plasmid induced changes in expression levels in E. coli
Citation(s)16432259;16981998;SeriesGSE1154
Samples
1:MG1655_replicate12:MG1655_replicate23:MG1655F_replicate1
4:MG1655F_replicate25:DH5alpha_replicate16:DH5alpha_replicate2
7:DH5alphaF_replicate18:DH5alphaF_replicate2
dsrA_b1954_st
more

External Database Connections

ECK120003633
EB4281
946470
ABE-0006490
EG31118
G7047
b1954
330
RF00014


Department of Biological Science and Technology,

Institute of Bioinformatics National Chiao Tung University, Hsinchu, Taiwan

Contact with: bryan@mail.NCTU.edu.tw